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Macro and Micronutrients Uncovered: Understanding Their Role, Deficiencies, and Clinical Relevance

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Macro and Micronutrients Uncovered: Understanding Their Role, Deficiencies, and Clinical Relevance

Did you know that most Americans struggle to meet their nutritional needs? Many do not maintain a nutritious diet, often overindulging in salt, unhealthy fats, and sweeteners, which heightens their chances of developing long-term health conditions. For instance, only a small fraction, less than 10%, of teenagers and adults intake the recommended amount of fruits and vegetables. (45)

Proper nutrition serves as a powerful tool for preventing and managing various health conditions, which is why nutrition is a cornerstone of functional medicine, deeply intertwined with the body's intricate mechanisms and overall well-being. 

A fundamental distinction lies in the differentiation between macronutrients and micronutrients. Macronutrients, encompassing proteins, carbohydrates, and fats, are the essential building blocks of our diet, providing the energy necessary for daily functions. On the other hand, micronutrients, which include vitamins and minerals, although required in smaller quantities, play equally vital roles in supporting numerous physiological processes. Recognizing the nuances between these two categories is essential for crafting personalized and effective nutritional strategies to ensure positive health outcomes.


What Are Macronutrients?

Macronutrients are fundamental components of the human diet that provide energy and contribute significantly to the structure and functioning of the body. Comprising carbohydrates, proteins, and fats, these nutrients provide the necessary energy and raw materials for essential biological processes, including metabolism, growth, and repair. (23


Carbohydrates are organic compounds that serve as the primary source of energy for the human body. Comprising carbon, hydrogen, and oxygen atoms, carbohydrates exist in various forms,  ranging from simple sugars to complex structures. Carbohydrates are classified into four major groups: monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Monosaccharides, encompassing glucose, fructose, and galactose, are the most basic units from which all carbohydrates are built. Two monosaccharides linked together form a disaccharide. Examples of disaccharides include sucrose (table sugar), lactose, and maltose. Oligosaccharides consist of three to six monosaccharide units. Polysaccharides are large molecules that consist of as many as thousands of linked monosaccharide units. Polysaccharides vary in size, sugar content, and structural complexity. (12

When consumed, carbohydrates undergo digestion, where enzymes break them down into their monosaccharide units, which are then absorbed through the small intestinal walls into the bloodstream. Carbohydrates act as a primary energy source in the body, help control blood sugar and insulin levels, participate in cholesterol and triglyceride metabolism, and support a healthy gut microbiome. Excess glucose not immediately required by cells is stored in the liver and muscles as glycogen until needed for energy. (19

The recommended daily intake of carbohydrates is about 45-65% of the total caloric intake. In nutrition, we refer to carbohydrates as being either simple or complex. Simple carbohydrates refer to those that contain one or two sugar molecules. They are quickly broken down and utilized by the body, providing a fast source of energy, and can lead to quick blood sugar spikes and dips. Foods high in simple carbohydrates tend to be low in fiber and high in added sugar, such as white bread and pastries, but are also found in fruits and milk products. (17

Complex carbohydrates are made of three or more monosaccharides. Their more complex chemical structures lead them to be broken down more slowly by the body, having a more gradual impact on blood sugar levels and enhancing satiety after eating. Complex carbohydrates are found in legumes, vegetables, and whole grains. (17


Proteins are complex molecules composed of amino acids linked together and folded into three-dimensional structures. There are 20 amino acids, 11 of which the body can synthesize, and nine (called essential amino acids) that must be obtained from diet. (22

Proteins are unfolded and broken down into their amino acid building blocks in the digestive tract. Amino acids are then absorbed through the small intestinal wall into the bloodstream to be transported to other body parts where they are required. Protein digestion is facilitated by digestive enzymes made by the stomach and pancreas. (22

Proteins serve a multitude of essential functions within the human body. Fundamentally, proteins act as the building blocks of life, constituting the structural basis of tissues, muscles, and organs. Enzymes, which are specialized proteins, facilitate and regulate various biochemical reactions, ensuring the body's metabolism operates efficiently. Proteins also play a vital role in immune function, defending the body against harmful invaders like viruses and bacteria. Additionally, proteins act as messengers, transmitting signals within and between cells, regulating biological processes, and ensuring proper communication in the nervous system. Hemoglobin, a protein in red blood cells, transports oxygen from the lungs to body tissues, enabling cellular respiration. Furthermore, proteins contribute to fluid balance, help maintain pH levels, and serve as a source of energy when carbohydrates and fats are insufficient. (22, 28

To prevent protein deficiency, the protein dietary reference intake (DRI) is 0.8 grams per kilogram of body weight per day. While these recommendations suffice for most healthy individuals, optimal protein intake varies based on age, physical activity level, and overall health. Athletes and bodybuilders, for instance, require higher protein intake to support muscle repair and growth due to the demands of their training regimes. Pregnant or breastfeeding women also need additional protein to support the development of the fetus or the production of breast milk. Similarly, elderly individuals might benefit from increased protein intake to prevent muscle loss associated with aging. For these special populations, protein needs may increase to 1.2-2 grams per kilogram of body weight per day. Protein-rich foods include meat, fish, eggs, dairy products, legumes, and nuts. (11


Dietary fat is the most energy-dense macronutrient made up of fatty acids and glycerol. Fats are essential for energy storage, insulation, cellular structure, nutrient absorption, brain health, and hormone production. Depending on their chemical structure, fats are categorized as saturated, unsaturated, or trans fats. (44

Saturated fats are dietary fat that consists of triglycerides in which the fatty acid chains lack double bonds between the individual carbon atoms. In simpler terms, the carbon atoms in the fatty acid chains are fully "saturated" with hydrogen atoms. This molecular structure gives saturated fats a stable and solid form at room temperature. Saturated fats are found in animal products like meat, poultry, and dairy items. They are also present in some plant oils, notably coconut and palm. Given that excessive intake of saturated fats has been linked to increased risk of heart disease, the American Heart Association (AHA) recommends limiting daily saturated fat intake to 5-6% of daily calories. (29, 44

Unsaturated fats are a type of dietary fat that is considered healthier than saturated fats due to their chemical structure. Unsaturated fats have one or more double bonds between the carbon atoms in the fatty acid chains. These double bonds create kinks in the chain, preventing the molecules from packing closely together. (44

There are two main types of unsaturated fats. Monounsaturated fats have one double bond in their fatty acid chain. Monounsaturated fats are typically found in high quantities in olive oil, avocados, nuts (e.g., almonds, peanuts, and cashews), and seeds (like sesame and pumpkin seeds). Polyunsaturated fats have multiple double bonds in their fatty acid chains. Polyunsaturated fats include two essential fatty acids: omega-3 fatty acids and omega-6 fatty acids. Sources of omega-3 fatty acids include fatty fish, flaxseeds, chia seeds, and walnuts. Omega-6 fatty acids are found in oils like soybean oil, corn oil, and sunflower oil. Both types of polyunsaturated fats are crucial for brain function, cell structure, and overall health. The daily DRI for fat is 20-35% of total calories, emphasizing healthy unsaturated fats. (29, 44

Trans fats are unsaturated fats that have been artificially manipulated through hydrogenation to enhance the shelf life and texture of foods. They are commonly found in fried foods, baked goods, margarine, snack foods, and processed and fast foods. High intake of trans fats has been linked to an increased risk of heart disease and high cholesterol. Due to these health risks, it is recommended to eliminate the intake of trans fats from the diet. (29)

What Are Micronutrients?

Micronutrients, encompassing vitamins and minerals, are required by the human body in relatively small amounts. Unlike macronutrients, micronutrients do not provide energy directly but are involved in various physiological processes essential for growth, development, and overall well-being. Deficiencies in micronutrients can lead to various health problems and diseases. Therefore, ensuring an adequate intake of vitamins and minerals is crucial for maintaining optimal health and preventing nutritional deficiencies. (24


Vitamins are organic compounds necessary for a wide range of biological functions, including immune support, energy production, and cell repair. There are two main types of vitamins: fat-soluble vitamins (such as vitamins A, D, E, and K) that are soluble in fats and oils, and water-soluble vitamins (such as vitamin C and various B vitamins) that dissolve in water. (43

Vitamin A

Vitamin A has several forms, including retinol, retinal, and beta-carotene. One of its primary roles lies in promoting healthy vision; it is crucial for the proper functioning of the retina's photoreceptor cells, allowing us to see in low light. Moreover, vitamin A is vital for supporting the immune system, aiding in the growth and development of cells, and maintaining the integrity of skin and mucous membranes. (34)

Vitamin D

Vitamin D, often called the "sunshine vitamin," promotes calcium absorption in the intestines and bone mineralization to reduce the risk of bone-related disorders such as osteoporosis and rickets. Beyond skeletal health, vitamin D plays a significant part in supporting the immune system, and emerging research suggests its involvement in regulating mood and protecting against certain chronic conditions, including heart disease and diabetes. While the body can produce vitamin D when exposed to sunlight, deficiency is common, especially in regions with limited sunlight, leading to the need for dietary supplementation or fortified foods to maintain optimal health. (25, 39

Vitamin E

Vitamin E is an antioxidant that exists in eight distinct chemical forms. It primarily protects cells from oxidative stress, serving as a powerful defense against the damaging effects of free radicals. By neutralizing these harmful molecules, vitamin E helps prevent cellular damage, reducing the risk of chronic diseases and supporting the immune system. Vitamin E also contributes to healthy skin and eyes, healing wounds, and maintaining vision. Moreover, it plays a role in maintaining a healthy cardiovascular system by preventing the oxidation of LDL cholesterol, reducing the risk of heart diseases. (40

Vitamin K

Vitamin K's primary role is in blood clotting, where it facilitates the production of proteins necessary for coagulation, preventing excessive bleeding from wounds and injuries. Beyond its role in coagulation, vitamin K is essential for bone health. It helps regulate calcium within the bones and blood vessels, ensuring proper mineralization of bone tissue and reducing the risk of osteoporosis and arterial calcification. (2

Vitamin C

Vitamin C, also known as ascorbic acid, is perhaps most renowned for its potent antioxidant properties. Vitamin C acts as a shield against harmful free radicals, protecting cells from oxidative stress. It plays a pivotal role in supporting the immune system, enhancing the body's ability to fight infections and illnesses. Additionally, vitamin C is vital for synthesizing collagen, a structural protein essential for skin, blood vessels, bones, and tendons. This makes vitamin C crucial for wound healing and maintaining healthy skin. Furthermore, it improves the absorption of non-heme iron (found in plant-based foods), helping to prevent iron deficiency anemia. (41

B Vitamins

B vitamins, including B1 (thiamine), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxine), B7 (biotin), B9 (folate), and B12 (cobalamin), collectively play indispensable roles in human health. These vitamins are essential for converting food into energy, supporting metabolism, and ensuring the proper functioning of the nervous system. B vitamins aid in synthesizing neurotransmitters, promoting healthy brain function and mood regulation. They are also crucial for DNA and red blood cell synthesis, contributing to optimal growth, repair, and development. Additionally, certain B vitamins regulate homocysteine levels, which, when elevated, can increase the risk of heart disease. Deficiencies in B vitamins can lead to various health issues, including anemia, fatigue, nerve damage, and developmental problems. (3


Minerals are inorganic elements that play diverse roles in the body, including bone formation, nerve signaling, muscle function, and enzyme regulation. Common minerals include calcium, iron, zinc, potassium, and magnesium.


Calcium provides structural support to bones and teeth, forming the mineral matrix that ensures their strength and integrity. Beyond skeletal health, calcium is crucial for muscle function, including the contraction and relaxation of muscles, as well as nerve signaling. Calcium is also involved in blood clotting, enabling the formation of blood clots to prevent excessive bleeding from wounds. (35


Iron is a key component of hemoglobin in red blood cells, enabling these cells to carry oxygen from the lungs to all body tissues and organs (1). Secondary to oxygen transport, iron aids in the synthesis of cellular energy (ATP), collagen, and thyroid hormones (5).  


Zinc helps maintain the integrity and function of the immune system, aiding the body in fighting off infections and illnesses. Zinc is also crucial for wound healing, promoting cell division and tissue repair. Moreover, it is involved in synthesizing DNA, RNA, and proteins, contributing to proper growth and development. Zinc is vital for the senses of taste and smell. Additionally, it plays a role in regulating mood and supporting cognitive function. (15, 37)


Potassium is one of the body's electrolytes, helping to maintain proper fluid balance within cells and optimal muscle and nerve function. Potassium plays a key role in regulating heartbeats and supporting cardiovascular health by helping the heart muscles contract and pump blood effectively. Moreover, it aids in balancing blood pressure, counteracting the adverse effects of sodium, and promoting healthy kidney function. Potassium also contributes to bone health by neutralizing acids in the body, reducing bone calcium loss. (36


Magnesium is a cofactor in over 300 enzymatic reactions in the body, including those involved in energy production, muscle contraction, and nerve impulse transmission. Magnesium also supports bone health by regulating calcium metabolism and bone density. (9

Common Causes for Nutrient Deficiencies

Major risk factors for nutrient deficiencies encompass a range of dietary, lifestyle, and health-related factors. Poor dietary choices, such as consuming diets high in processed foods, sugars, and unhealthy fats while lacking nutrient-dense fruits, vegetables, and whole grains, can lead to deficiencies in vitamins and minerals. Restrictive diets, like veganism or extreme calorie restrictions, may also lack certain nutrients. Medical conditions such as malabsorption disorders can hinder the body's ability to absorb and utilize nutrients effectively. Moreover, socioeconomic factors, including poverty and limited access to nutritious foods, can contribute to deficiencies. Age-related factors, such as increased nutritional needs during growth phases or decreased absorption abilities in the elderly, also pose risks. Pregnancy and lactation demand higher nutrient intake, and certain medications can interfere with nutrient absorption. 

Recognizing the Signs of Deficiency

Despite the country's overall affluence and food availability, nutritional deficiency and inadequacy remain a concern in the United States. The NHANES 2007-2010 survey, covering 16,444 individuals aged four and above, revealed widespread micronutrient inadequacies among Americans. The data indicated that a significant portion of the population did not meet the daily requirements for essential nutrients, with inadequacies notably high for vitamin D (94.3%), vitamin E (88.5%), magnesium (52.2%), calcium (44.1%), vitamin A (43.0%), and vitamin C (38.9%). (16

Nutrient deficiencies can manifest in various ways, leading to distinctive signs and symptoms. Examples are listed below:

  • Vitamin D: bone pain and muscle weakness
  • Vitamin E: neuropathy, vision and balance problems, impaired immune responses
  • Vitamin C: fatigue, bleeding gums, and frequent infections
  • Vitamin A: night blindness and impaired immune function
  • Zinc: impaired immune function, delayed wound healing, loss of appetite, decreased taste and smell
  • Iron: fatigue, pale skin, and shortness of breath

Additionally, deficiencies and inadequacies are linked to the onset of progression of various chronic diseases, like cancer, cardiovascular disease, type 2 diabetes, osteoporosis, and age-related eye disease (16). 

How to Use Functional Medicine Labs to Evaluate Macro and Micronutrient Status

Functional medicine labs are valuable tools for assessing macro and micronutrient status, providing detailed insights into an individual's health.

Micronutrient Testing

A micronutrient test is a comprehensive blood test designed to evaluate the levels of various essential vitamins, minerals, antioxidants, amino acids, and fatty acids in the body. By analyzing these micronutrient levels, healthcare professionals can tailor personalized dietary recommendations and supplementation plans to address deficiencies, support overall health, and prevent chronic diseases associated with nutritional imbalances.

Organic Acid Testing

The Organic Acid Test (OAT) is a diagnostic tool used to assess nutritional status and overall health by analyzing the organic acids present in urine. These organic acids are metabolic intermediates produced during various biochemical pathways within the body. By examining these compounds, the OAT can provide valuable insights into nutrient metabolism, energy production, neurotransmitter function, and oxidative stress. Regarding nutritional status, the OAT can detect deficiencies in specific vitamins and minerals. For example, it can reveal markers associated with vitamin B12, B6, and CoQ10 deficiencies, as well as imbalances in amino acids and fatty acids. Additionally, it can identify problems related to the metabolism of carbohydrates, indicating issues like insulin resistance. The Organic Acid Test (OAT) by Mosaic Diagnostics is a great example of an organic acid test.

Therapeutic Interventions and Personalized Nutrition Plans

Personalized nutrition plans are pivotal in preventing and addressing macro and micronutrient deficiencies as they consider an individual's unique dietary preferences, health status, and lifestyle. For macronutrients, a tailored approach might involve adjusting the balance of carbohydrates, proteins, and fats to meet specific needs and health goals. For instance, athletes may require higher protein intake for muscle repair, while those with insulin resistance and elevated blood sugar might benefit from controlled carbohydrate intake.

Addressing micronutrient deficiencies involves dietary changes and targeted supplements. For vitamin and mineral deficiencies, recommending foods rich in the specific nutrients of concern is essential. However, additional supplements are sometimes required to correct and prevent deficiencies. For instance, individuals with vitamin D deficiency might need supplements, especially during winter months. Likewise, vegans and vegetarians may need supplemental vitamin B12. (26)

Personalized nutrition plans also consider allergies, intolerances, and cultural dietary habits. For example, someone lactose intolerant might be advised to eat non-dairy calcium sources. Continuous monitoring and adjustments to these plans, based on regular health assessments and follow-ups, ensure that individuals receive the necessary nutrients tailored to their specific needs, promoting optimal health and preventing deficiencies. Consulting a registered dietitian or healthcare professional for personalized guidance is essential in developing effective and sustainable dietary and supplemental interventions. (26)



Understanding the intricate balance of macro and micronutrients is paramount in clinical nutrition, one of the pillars of optimal health. A holistic approach rooted in functional medicine principles offers comprehensive insights into nutrient balance and its impact on overall well-being. Grasping the connection between nutrient deficiencies and chronic diseases, as well as timely recognition of nutritional imbalances, is vital for preventive and holistic healthcare. For guidance tailored to individual needs and health goals, consulting with a functional medicine practitioner to discuss a personalized nutrition assessment is highly recommended. 

The information provided is not intended to be a substitute for professional medical advice. Always consult with your doctor or other qualified healthcare provider before taking any dietary supplement or making any changes to your diet or exercise routine.
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