Subscribe to the Magazine for free.
Subscribe for free to keep reading! If you are already subscribed, enter your email address to log back in.
Thanks for subscribing!
Oops! Something went wrong while submitting the form.
Are you a healthcare practitioner?
Thanks for subscribing!
Oops! Something went wrong while submitting the form.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.

How to Use the Metabolomix+ in Practice for Best Patient Outcome

Medically reviewed by 
How to Use the Metabolomix+ in Practice for Best Patient Outcome

Functional doctors know the importance nutrition plays in the body. Nutrient deficiencies commonly play a role in disease manifestation, whether they contribute to disease development or are an unwanted consequence of the disease process. Conventional labs offer limited, incomplete testing options to assess nutritional status. For those seeking nutritional guidance specific to optimizing health and preventing disease, specialty labs offer comprehensive nutritional panels that can identify micro- and macronutrient imbalances and guide personalized dietary recommendations.

Metabolomix+ is a panel offered through Genova Diagnostics that assesses nutritional status based on the functional assessment of organic acids, oxidative stress markers, and amino acids. This non-invasive test is easy for patients to collect at home and features a user-friendly report easily interpreted to direct individualized nutritional recommendations.


What is The Metabolomix+ Test

The Metabolomix+ is a comprehensive and functional nutritional evaluation of organic acids and amino acids used to screen for antioxidant, vitamin, mineral, and protein insufficiencies. It is a non-invasive test requiring a single urine collection, which can be collected in the comfort of the patient's home.

Options for an at-home blood spot finger stick and/or cheek swab allow the provider to add measurements for fatty acids, toxic element exposure, 15 additional nutrients, and four common genetic polymorphisms onto the test.

The Metabolomix+ is very similar to Genova's NutrEval FMV panel, but includes fewer analytes, is lower in cost, and does not require a blood draw.

Unique features of Genova test reports include:

  • Results Overview with functional imbalance scores to easily interpret patient results and prioritize treatment recommendations that address methylation, toxic exposure, mitochondrial dysfunction, fatty acid imbalance, and oxidative stress
  • Nutrient Need Overview page that includes personalized recommendations for antioxidants, vitamins, minerals, essential fatty acids, amino acids, and digestive support
  • Interpretation-At-A-Glance provides education on functional nutritional therapy for the doctor and the patient.

What Does The Metabolomix+ Test Measure

The basic Metabolomix+ (without add-on components) measures 93 analytes related to nutritional status. The test report is broken down into six metabolic areas:

Organic Acids are metabolic byproducts of the cells that can act as markers to assess:

  • Digestive function
  • Intestinal bacterial and yeast dysbiosis
  • Mitochondrial function and cellular energy production
  • Neurotransmitter synthesis and metabolism
  • Oxalate production
  • Toxic load and antioxidant levels
  • Vitamin levels

Oxidative Stress Markers assist in quantifying the level of cellular and DNA damage present, which can indicate the need for antioxidant support.

Amino Acids measured in urine reflect recent protein intake over the last 48-72 hours. Essential and non-essential amino acids provide insight into protein and amino acid intake, digestion/absorption, and synthesis. Other markers in this section give additional information regarding the nutritional status of vitamin cofactors required for protein metabolism.

Essential and Metabolic Fatty Acid Markers (Optional Add-On) measure the patient's omega-3, omega-6, omega-9, saturated, and monounsaturated fatty acid levels.

Nutrient and Toxic Elements (Optional Add-On) assess an additional 20 toxic and 15 mineral elements.

Genetic SNPs (Optional Add-On) are variations in the genetic code that may influence an individual's biochemistry and physiology and increase the risk for disease. SNPs that can be added to this test include MTHFR, COMT, TNF-a, and APOE.

When to Consider The Metabolomix+ Test

Nutrient insufficiencies/deficiencies can negatively affect every body system and contribute to disease processes. Functional nutritional analysis is most commonly recommended for the following clinical conditions:

Nutritional assessment is also an excellent tool for patients needing additional dietary guidance. This may include patients with histories of restricted eating patterns, those looking to gain or lose weight, and those pursuing to optimize athletic performance.

Finally, nutritional assessments can be ordered for patients without health concerns but looking to prevent disease. By proactively assessing metabolic function and nutritional status, you can avoid nutritional insufficiency, disease, and associated symptoms through dietary modifications and supplemental protocols.

How to Use the Metabolomix+ in Practice for Best Patient Outcome

This test cannot be ordered for patients under two years.

Patients should be advised on proper sample collection protocol before test collection to ensure valid test results. Complete patient instructions can be found on Rupa Health's website. Key details include:

  • Do not collect urine sample while menstruating
  • Limit fluid intake to 48 ounces 24 hours before sample collection
  • Fast overnight (water ok) before sample collection. Collect samples immediately upon waking before eating or drinking anything (water ok).

The decision to advise your patient to discontinue medications and supplements prior to sample collection depends on the risk associated with discontinuation and what you hope to achieve with the test. Diuretics, valproic acid, acetaminophen (Tylenol), and berberine may affect test results but should not be discontinued if they are essential to the patient's health. Non-essential supplements should be discontinued at least four days prior to sample collection if wanting to assess the patient's baseline nutritional status. You can monitor the efficacy of and patient response to nutritional therapy by advising your patient to continue their supplemental regimen leading up to the specimen collection.

This test's optional fatty acid component can be exceptionally beneficial for patients in whom you are looking to decrease inflammation, support brain/nerve function, and reduce cardiovascular risk.

The results of this report should guide personalized nutritional recommendations through diet and supplements to correct insufficiencies/deficiencies identified on the panel. Antioxidant support, herbal antimicrobials, and digestive enzymes may also be indicated to support detoxification and digestion and to correct intestinal dysbiosis.

The results of Metabolomix+ may indicate the need for additional testing. Genova tests frequently ordered in conjunction with Metabolomix+ include:


Genova offers comprehensive functional nutritional testing through its Metabolomix+ profile. By direct and functional assessment of organic acids, amino acids, fatty acids, vitamins, and minerals, this test can help identify imbalances related to common health concerns and diseases. Optional add-ons allow you to customize the test to the patient's needs. The results of this test will provide insight into how nutritional and environmental factors impact your patients, guide personalized nutritional treatment recommendations, and may expose the need for additional testing. Metabolomix+ is a cost-effective, easy-to-collect test for patients with a wide range of clinical applicability.

The information provided is not intended to be a substitute for professional medical advice. Always consult with your doctor or other qualified healthcare provider before taking any dietary supplement or making any changes to your diet or exercise routine.
Learn More
No items found.

Lab Tests in This Article


Subscribe to the Magazine for free. to keep reading!
Subscribe for free to keep reading, If you are already subscribed, enter your email address to log back in.
Thanks for subscribing!
Oops! Something went wrong while submitting the form.
Are you a healthcare practitioner?
Thanks for subscribing!
Oops! Something went wrong while submitting the form.