Lab Education
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March 5, 2024

Understanding and Interpreting Methylation Tests: A Beginner's Guide

Medically Reviewed by
Updated On
September 17, 2024

Methylation is a crucial biochemical process that affects how the body functions. It plays a fundamental role in DNA repair, gene expression, and detoxification by chemically adding methyl groups to various molecules, such as DNA and proteins. This addition influences gene activity, determining which genes are active or inactive at any given time.

Imbalances in methylation can lead to a spectrum of health issues. Enter methylation tests– these tools can offer insights into how our genes interact with our environment, diet, and lifestyle choices. This information can help us to better understand potential disease risks and guide personalized strategies for prevention and treatment.

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The Basics of Methylation

Methylation involves adding a methyl group, composed of one carbon atom and three hydrogen atoms, to certain molecules, impacting a wide variety of cellular processes. Specifically, methylation of DNA molecules regulates gene expression and various cellular functions.

Not all genes are active at all times. Cells utilize DNA methylation as a regulatory mechanism to control gene expression, acting as a switch to turn genes “off” or “on.” The addition of methyl groups typically results in gene silencing, while their removal activates previously silenced genes. This phenomenon is a key aspect of epigenetics– a discipline exploring how cells control gene activity without altering the DNA sequence. The enzymes responsible for facilitating DNA methylation are called DNA methyltransferases (DNMTs).

Maintaining a delicate balance of methylation is crucial for cellular homeostasis (stability).  Disruptions in this balance, often associated with lifestyle factors or genetic predispositions, can negatively impact cellular functions, contributing to the development of various diseases.

Defects in methylation and epigenetic processes lead to abnormal gene expression, resulting in significant health implications. Methylation issues have been linked to conditions such as cancer, cardiovascular disease, diabetes, and neurodegenerative disorders.

What Are Methylation Tests?

Methylation tests are assessments designed to evaluate the efficiency and functionality of the methylation process within the body. These tests measure the levels of specific biomarkers associated with methylation, detecting abnormalities and providing insights into the regulation of gene expression and overall cellular health. Various methylation tests, including DNA methylation tests and homocysteine testing, are available.

Mosaic Diagnostics’ DNA Methylation Pathway Profile, for example, screens for single nucleotide polymorphisms (SNPs) or genetic changes that may impact metabolism, detoxification, hormone balance, and vitamin D function. Influenced by epigenetic factors like diet, lifestyle, nutrition, and toxin exposures, these SNPs can guide treatment plans to address and prevent associated diseases. Genova Diagnostics also offers a Methylation Panel, including both SNPs and methylation metabolites, with recommendations for nutritional support such as amino acids, vitamins, and minerals.

Homocysteine testing, offered by BostonHeart Diagnostics and other labs, provides another perspective on methylation. Homocysteine, an amino acid, is broken down by vitamins B12, B6, and folate to create other substances the body needs. Elevated blood homocysteine levels can indicate a deficiency in one or more of these vitamins, or a mutation in the methylenetetrahydrofolate reductase gene (MTHFR), increasing the risk of heart disease, stroke, and dementia.

These tests are valuable tools in personalized medicine, offering information about an individual's unique methylation profile. Healthcare professionals can tailor interventions to address particular health risks and optimize overall well-being by identifying imbalances or deficiencies in the methylation process. 

[signup]

Indications for Methylation Testing

Methylation testing may be useful in various scenarios, particularly when individuals exhibit symptoms such as chronic fatigue or mood disorders or have a family history of certain diseases.

Chronic fatigue, characterized by persistent and unexplained tiredness that is not alleviated with rest, may prompt healthcare providers to assess methylation status due to its impact on energy production.

Mood disorders, encompassing depression, anxiety, ADHD, bipolar disorder, and schizophrenia, indicate potential disturbances in brain chemical regulation associated with methylation. In bipolar disorder (BD), research highlights the link between alterations in neurotransmitters during mood episodes and DNA methylation abnormalities in genes related to the dopamine system, such as catechol-O-methyltransferase (COMT), suggesting potential links to gene expression changes.  Similarly, depression has been associated with DNA methylation defects in genes like brain-derived neurotrophic factor (BDNF) and serotonin transporter (SERT).

Moreover, a family history of diseases related to methylation, such as cardiovascular conditions or neurodegenerative disorders, may prompt healthcare professionals to recommend these tests.

Other methylation testing indications may include concerns with detoxification function, cognitive decline, and cancer risk. These tests, integral to personalized medicine and preventive healthcare, offer valuable insights into an individual's unique methylation profile. By addressing specific imbalances or deficiencies, individuals can manage symptoms and proactively reduce disease risks.

How to Prepare for a Methylation Test

Preparing for a methylation test involves several considerations to ensure accurate and meaningful results. First and foremost, individuals should follow their healthcare provider's instructions and adhere to guidelines provided by the laboratory conducting the test. Each type of test may have different recommendations. For example, while salivary tests may not require any specific preparation, blood tests have more detailed instructions.

Dietary restrictions are often involved in methylation testing preparation. Overnight fasting for 8-12 hours is typically required prior to blood draws. To avoid influencing methylation processes, individuals may need to avoid specific foods or supplements, such as amino acid supplements or “methyl donors” like S-adenosyl-L-methionine (SAMe). It is equally important to consider pharmaceutical medications. It is always advised to consult with your personal physician regarding substances that may impact the methylation cycle to avoid skewing results. In some cases, healthcare providers may recommend temporarily discontinuing certain medications before the test.

Timing is also crucial for accurate results. Certain methylation tests may require samples to be taken in the morning or under fasting conditions to ensure standardized results and reliability. If shipping samples to a lab, ensuring an ice pack is adequately prepared prior to collection may be necessary. Always read sample collection instructions thoroughly and well before the intended collection time.

Preparing for a methylation test involves clear communication with your healthcare provider, adherence to timing guidelines and dietary restrictions, and careful consideration of medication and supplement influences.

Interpreting Methylation Test Results

Accurately interpreting methylation test results is crucial for gaining insights into one's health and identifying potential risk factors. Genetic mutations and abnormal methylation patterns may indicate the need for further investigation and intervention.

DNA methylation tests can reveal genetic mutations. For instance, we all have two MTHFR genes, one from each parent. The presence of a mutation in one gene is termed heterozygous, while mutations in both genes indicate homozygosity, leading to more impaired enzyme function. Detecting these genetic mutations before symptoms emerge allows for proactive preventive measures. While some sources advise that no intervention is necessary, others propose the potential benefits of dietary supplements and nutritional strategies.

Abnormal methylation patterns, such as elevated homocysteine levels, may indicate an increased risk for conditions like cardiovascular disease, neurodegenerative disorders, or hormonal imbalances. Depending on the results, healthcare providers may recommend additional diagnostic tests, such as B vitamin assessments, to delve deeper into specific health concerns.

Test results can guide personalized treatment plans, assisting healthcare professionals in making targeted recommendations for dietary changes or supplementation to address identified imbalances. Lifestyle factors, including physical activity, smoking, and alcohol consumption, can influence methylation processes. Incorporating positive changes in these areas can optimize epigenetic patterns and promote overall well-being.

Understanding methylation status can aid in shaping prevention strategies. Identifying potential health risks through methylation testing empowers individuals to proactively address underlying issues before symptoms manifest, emphasizing the significance of preventive healthcare.

Potential Interventions Based on Methylation Status

Addressing methylation imbalances requires a holistic approach, often involving a combination of dietary supplements and lifestyle modifications.

B Vitamins

B vitamin supplementation, particularly cobalamin (B12), folate (B9), and pyridoxine (B6), is a common recommendation to support methylation and prevent abnormal gene expression. When supplementing with B12, opting for the methylated form (methylcobalamin) over cyanocobalamin is recommended for enhanced absorption and efficacy. Riboflavin (B2), choline, methionine, and betaine are additional vital nutrients for methylation, found in foods like lean animal proteins, eggs, cruciferous vegetables, whole grains, nuts, and seeds.

Folate

Folate is particularly important for methylation, as it provides methyl groups for cellular processes. Ensuring sufficient folate intake, obtained through diet or supplementation, supports healthy methylation. Particularly during pregnancy, low folate and B12 levels are linked to neural tube defects and many other health issues. Therefore, folate supplementation is essential for individuals with mutations impairing methylation pathways. The methylated form (methylfolate) is recommended. Dietary sources of folate include leafy greens and legumes.

Lifestyle

Comprehensive methylation support extends to lifestyle modifications, such as regular physical activity and a balanced, nutrient-rich diet. It is also advised to harmful habits like smoking and excessive alcohol consumption, as these have negative impacts on methylation. 

The Limitations of Methylation Testing

Methylation testing, while valuable, presents several limitations that warrant consideration. A notable challenge lies in the variability across testing methods employed by different laboratories, introducing possible inconsistencies in results. Standardization in methodologies is lacking, which may contribute to potential discrepancies in interpretations. The accuracy of these tests, as reflected by sensitivity and specificity, also remains unclear, raising questions about their reliability.

Furthermore, the interpretation of methylation test results can be complex given the dynamic nature of epigenetic processes, the interplay of genetic and environmental factors, and variations in individual responses to methylation changes. The evolving understanding of the functional significance of specific methylation patterns and the limited availability of DNA methylation datasets further complicate drawing definitive conclusions from test outcomes.

To navigate these testing limitations effectively, healthcare professionals should employ comprehensive assessments, considering not only test results but also the patient’s clinical presentation, medical history, and other diagnostic findings. A holistic perspective and integrating results into the broader clinical context are imperative for informed decision-making.

[signup]

Key Takeaways

Methylation, a critical biochemical process governing DNA repair, gene expression, and detoxification, is fundamental to our health. Advanced tests offer insights into methylation patterns and individual genetic interactions with lifestyle and environment. This information may be used to guide personalized wellness strategies, including nutrition, lifestyle, and interventions with dietary supplements.

When considering methylation tests, it is important to understand their implications, limitations, and the need for comprehensive assessments. Consulting a healthcare professional is recommended to determine if methylation testing is appropriate for you. These tests function as diagnostic allies and are especially useful in cases such as chronic fatigue, mood disorders, or familial health concerns. Early identification of potential imbalances empowers individuals to proactively manage risk factors, ultimately optimizing their health.

Methylation is a crucial biochemical process that affects how the body functions. It plays a fundamental role in DNA repair, gene expression, and detoxification by chemically adding methyl groups to various molecules, such as DNA and proteins. This addition influences gene activity, determining which genes are active or inactive at any given time.

Imbalances in methylation can lead to a spectrum of health issues. Methylation tests can offer insights into how our genes interact with our environment, diet, and lifestyle choices. This information can help us to better understand potential health risks and guide personalized strategies for wellness and health maintenance.

[signup]

The Basics of Methylation

Methylation involves adding a methyl group, composed of one carbon atom and three hydrogen atoms, to certain molecules, impacting a wide variety of cellular processes. Specifically, methylation of DNA molecules regulates gene expression and various cellular functions.

Not all genes are active at all times. Cells utilize DNA methylation as a regulatory mechanism to control gene expression, acting as a switch to turn genes “off” or “on.” The addition of methyl groups typically results in gene silencing, while their removal activates previously silenced genes. This phenomenon is a key aspect of epigenetics– a discipline exploring how cells control gene activity without altering the DNA sequence. The enzymes responsible for facilitating DNA methylation are called DNA methyltransferases (DNMTs).

Maintaining a delicate balance of methylation is crucial for cellular homeostasis (stability). Disruptions in this balance, often associated with lifestyle factors or genetic predispositions, can negatively impact cellular functions, contributing to the development of various health concerns.

Defects in methylation and epigenetic processes lead to abnormal gene expression, resulting in significant health implications. Methylation issues have been linked to conditions such as cancer, cardiovascular disease, diabetes, and neurodegenerative disorders.

What Are Methylation Tests?

Methylation tests are assessments designed to evaluate the efficiency and functionality of the methylation process within the body. These tests measure the levels of specific biomarkers associated with methylation, detecting abnormalities and providing insights into the regulation of gene expression and overall cellular health. Various methylation tests, including DNA methylation tests and homocysteine testing, are available.

Mosaic Diagnostics’ DNA Methylation Pathway Profile, for example, screens for single nucleotide polymorphisms (SNPs) or genetic changes that may impact metabolism, detoxification, hormone balance, and vitamin D function. Influenced by epigenetic factors like diet, lifestyle, nutrition, and toxin exposures, these SNPs can guide wellness plans to address and support health. Genova Diagnostics also offers a Methylation Panel, including both SNPs and methylation metabolites, with recommendations for nutritional support such as amino acids, vitamins, and minerals.

Homocysteine testing, offered by BostonHeart Diagnostics and other labs, provides another perspective on methylation. Homocysteine, an amino acid, is broken down by vitamins B12, B6, and folate to create other substances the body needs. Elevated blood homocysteine levels can indicate a deficiency in one or more of these vitamins, or a mutation in the methylenetetrahydrofolate reductase gene (MTHFR), which may be associated with certain health risks.

These tests are valuable tools in personalized wellness, offering information about an individual's unique methylation profile. Healthcare professionals can tailor interventions to address particular health risks and optimize overall well-being by identifying imbalances or deficiencies in the methylation process.

[signup]

Indications for Methylation Testing

Methylation testing may be useful in various scenarios, particularly when individuals exhibit symptoms such as chronic fatigue or mood disorders or have a family history of certain health conditions.

Chronic fatigue, characterized by persistent and unexplained tiredness that is not alleviated with rest, may prompt healthcare providers to assess methylation status due to its impact on energy production.

Mood disorders, encompassing depression, anxiety, ADHD, bipolar disorder, and schizophrenia, indicate potential disturbances in brain chemical regulation associated with methylation. In bipolar disorder (BD), research highlights the link between alterations in neurotransmitters during mood episodes and DNA methylation abnormalities in genes related to the dopamine system, such as catechol-O-methyltransferase (COMT), suggesting potential links to gene expression changes. Similarly, depression has been associated with DNA methylation defects in genes like brain-derived neurotrophic factor (BDNF) and serotonin transporter (SERT).

Moreover, a family history of health conditions related to methylation, such as cardiovascular conditions or neurodegenerative disorders, may prompt healthcare professionals to recommend these tests.

Other methylation testing indications may include concerns with detoxification function, cognitive decline, and cancer risk. These tests, integral to personalized wellness and preventive healthcare, offer valuable insights into an individual's unique methylation profile. By addressing specific imbalances or deficiencies, individuals can manage symptoms and proactively support their health.

How to Prepare for a Methylation Test

Preparing for a methylation test involves several considerations to ensure accurate and meaningful results. First and foremost, individuals should follow their healthcare provider's instructions and adhere to guidelines provided by the laboratory conducting the test. Each type of test may have different recommendations. For example, while salivary tests may not require any specific preparation, blood tests have more detailed instructions.

Dietary restrictions are often involved in methylation testing preparation. Overnight fasting for 8-12 hours is typically required prior to blood draws. To avoid influencing methylation processes, individuals may need to avoid specific foods or supplements, such as amino acid supplements or “methyl donors” like S-adenosyl-L-methionine (SAMe). It is equally important to consider pharmaceutical medications. It is always advised to consult with your personal physician regarding substances that may impact the methylation cycle to avoid skewing results. In some cases, healthcare providers may recommend temporarily discontinuing certain medications before the test.

Timing is also crucial for accurate results. Certain methylation tests may require samples to be taken in the morning or under fasting conditions to ensure standardized results and reliability. If shipping samples to a lab, ensuring an ice pack is adequately prepared prior to collection may be necessary. Always read sample collection instructions thoroughly and well before the intended collection time.

Preparing for a methylation test involves clear communication with your healthcare provider, adherence to timing guidelines and dietary restrictions, and careful consideration of medication and supplement influences.

Interpreting Methylation Test Results

Accurately interpreting methylation test results is crucial for gaining insights into one's health and identifying potential risk factors. Genetic mutations and abnormal methylation patterns may indicate the need for further investigation and intervention.

DNA methylation tests can reveal genetic mutations. For instance, we all have two MTHFR genes, one from each parent. The presence of a mutation in one gene is termed heterozygous, while mutations in both genes indicate homozygosity, leading to more impaired enzyme function. Detecting these genetic mutations before symptoms emerge allows for proactive preventive measures. While some sources advise that no intervention is necessary, others propose the potential benefits of dietary supplements and nutritional strategies.

Abnormal methylation patterns, such as elevated homocysteine levels, may indicate an increased risk for conditions like cardiovascular disease, neurodegenerative disorders, or hormonal imbalances. Depending on the results, healthcare providers may recommend additional diagnostic tests, such as B vitamin assessments, to delve deeper into specific health concerns.

Test results can guide personalized wellness plans, assisting healthcare professionals in making targeted recommendations for dietary changes or supplementation to address identified imbalances. Lifestyle factors, including physical activity, smoking, and alcohol consumption, can influence methylation processes. Incorporating positive changes in these areas can optimize epigenetic patterns and promote overall well-being.

Understanding methylation status can aid in shaping prevention strategies. Identifying potential health risks through methylation testing empowers individuals to proactively address underlying issues before symptoms manifest, emphasizing the significance of preventive healthcare.

Potential Interventions Based on Methylation Status

Addressing methylation imbalances requires a holistic approach, often involving a combination of dietary supplements and lifestyle modifications.

B Vitamins

B vitamin supplementation, particularly cobalamin (B12), folate (B9), and pyridoxine (B6), is a common recommendation to support methylation and maintain normal gene expression. When supplementing with B12, opting for the methylated form (methylcobalamin) over cyanocobalamin is suggested for enhanced absorption and efficacy. Riboflavin (B2), choline, methionine, and betaine are additional vital nutrients for methylation, found in foods like lean animal proteins, eggs, cruciferous vegetables, whole grains, nuts, and seeds.

Folate

Folate is particularly important for methylation, as it provides methyl groups for cellular processes. Ensuring sufficient folate intake, obtained through diet or supplementation, supports healthy methylation. Particularly during pregnancy, low folate and B12 levels are linked to neural tube defects and many other health issues. Therefore, folate supplementation is essential for individuals with mutations impacting methylation pathways. The methylated form (methylfolate) is recommended. Dietary sources of folate include leafy greens and legumes.

Lifestyle

Comprehensive methylation support extends to lifestyle modifications, such as regular physical activity and a balanced, nutrient-rich diet. It is also advised to avoid harmful habits like smoking and excessive alcohol consumption, as these have negative impacts on methylation.

The Limitations of Methylation Testing

Methylation testing, while valuable, presents several limitations that warrant consideration. A notable challenge lies in the variability across testing methods employed by different laboratories, introducing possible inconsistencies in results. Standardization in methodologies is lacking, which may contribute to potential discrepancies in interpretations. The accuracy of these tests, as reflected by sensitivity and specificity, also remains unclear, raising questions about their reliability.

Furthermore, the interpretation of methylation test results can be complex given the dynamic nature of epigenetic processes, the interplay of genetic and environmental factors, and variations in individual responses to methylation changes. The evolving understanding of the functional significance of specific methylation patterns and the limited availability of DNA methylation datasets further complicate drawing definitive conclusions from test outcomes.

To navigate these testing limitations effectively, healthcare professionals should employ comprehensive assessments, considering not only test results but also the patient’s clinical presentation, medical history, and other diagnostic findings. A holistic perspective and integrating results into the broader clinical context are imperative for informed decision-making.

[signup]

Key Takeaways

Methylation, a critical biochemical process governing DNA repair, gene expression, and detoxification, is fundamental to our health. Advanced tests offer insights into methylation patterns and individual genetic interactions with lifestyle and environment. This information may be used to guide personalized wellness strategies, including nutrition, lifestyle, and interventions with dietary supplements.

When considering methylation tests, it is important to understand their implications, limitations, and the need for comprehensive assessments. Consulting a healthcare professional is recommended to determine if methylation testing is appropriate for you. These tests function as diagnostic allies and are especially useful in cases such as chronic fatigue, mood disorders, or familial health concerns. Early identification of potential imbalances empowers individuals to proactively manage risk factors, ultimately optimizing their health.

The information in this article is designed for educational purposes only and is not intended to be a substitute for informed medical advice or care. This information should not be used to diagnose or treat any health problems or illnesses without consulting a doctor. Consult with a health care practitioner before relying on any information in this article or on this website.

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Lab Tests in This Article

1. Cecil, C. A. M., & Nigg, J. T. (2022). Epigenetics and ADHD: Reflections on Current Knowledge, Research Priorities and Translational Potential. Molecular diagnosis & therapy, 26(6), 581–606. https://doi.org/10.1007/s40291-022-00609-y

2. Christie, J. (2023, February 15). Testing B Vitamin Levels: What You Need to Know. Rupa Health. https://www.rupahealth.com/post/testing-b-vitamin-levels-what-you-need-to-know

3. Cloyd, J. (2024, February 27). Exploring Neurotransmitter Testing: A Functional Medicine Perspective on Mental Health. Rupa Health. https://www.rupahealth.com/post/exploring-neurotransmitter-testing-a-functional-medicine-perspective-on-mental-health

4. DNA Methylation Pathway Profile | Rupa Health Patient Help Center. (n.d.). Support.rupahealth.com. Retrieved March 1, 2024, from https://support.rupahealth.com/en/articles/8552228-dna-methylation-pathway-profile

5. DNA Methylation Pathway Profile by Mosaic Diagnostics (formerly Great Plains). (n.d.). Rupa Health. Retrieved March 1, 2024, from https://www.rupahealth.com/lab-tests/mosaic-diagnostics-dna-methylation-pathway-profile#description

6. Ebrahimpour, A., Vaghari-Tabari, M., Qujeq, D., Moein, S., & Moazezi, Z. (2018). Direct correlation between serum homocysteine level and insulin resistance index in patients with subclinical hypothyroidism: Does subclinical hypothyroidism increase the risk of diabetes and cardiovascular disease together?. Diabetes & metabolic syndrome, 12(6), 863–867. https://doi.org/10.1016/j.dsx.2018.05.002

7. Genetic Test: COMT by Ayumetrix. (n.d.). Rupa Health. Retrieved March 1, 2024, from https://www.rupahealth.com/lab-tests/ayumetrix-genetic-test-comt#description

8. Gunter, C. (2024, February 29). Single Nucleotide Polymorphisms (SNPs). Genome.gov. https://www.genome.gov/genetics-glossary/Single-Nucleotide-Polymorphisms#:~:text=A%20single%20nucleotide%20polymorphism%20

9. Helliwell, A. M., Sweetman, E. C., Stockwell, P. A., Edgar, C. D., Chatterjee, A., & Tate, W. P. (2020). Changes in DNA methylation profiles of myalgic encephalomyelitis/chronic fatigue syndrome patients reflect systemic dysfunctions. Clinical Epigenetics, 12(1), 167. https://doi.org/10.1186/s13148-020-00960-z

10. Homocysteine by BostonHeart Diagnostics. (n.d.). Rupa Health. Retrieved March 1, 2024, from https://www.rupahealth.com/lab-tests/bostonheart-homocysteine

11. Kim M. (2019). DNA methylation: a cause and consequence of type 2 diabetes. Genomics & informatics, 17(4), e38. https://doi.org/10.5808/GI.2019.17.4.e38

12. Klemp, I., Hoffmann, A., Müller, L., Hagemann, T., Horn, K., Rohde-Zimmermann, K., Tönjes, A., Thiery, J., Löffler, M., Burkhardt, R., Böttcher, Y., Stumvoll, M., Blüher, M., Krohn, K., Scholz, M., Baber, R., Franks, P. W., Kovacs, P., & Keller, M. (2022). DNA methylation patterns reflect individual's lifestyle independent of obesity. Clinical and translational medicine, 12(6), e851. https://doi.org/10.1002/ctm2.851

13. Kresge, K. (2022, April 22). 5 Micronutrient Abnormalities That May Make Bipolar Disorder Worse. Rupa Health. https://www.rupahealth.com/post/a-functional-medicine-approach-to-bipolar-disorder

14. Kurdyukov, S., & Bullock, M. (2016). DNA Methylation Analysis: Choosing the Right Method. Biology, 5(1), 3. https://doi.org/10.3390/biology5010003

15. Lakshminarasimhan, R., & Liang, G. (2016). The Role of DNA Methylation in Cancer. Advances in experimental medicine and biology, 945, 151–172. https://doi.org/10.1007/978-3-319-43624-1_7

16. Lanata, C. M., Chung, S. A., & Criswell, L. A. (2018). DNA methylation 101: what is important to know about DNA methylation and its role in SLE risk and disease heterogeneity. Lupus science & medicine, 5(1), e000285. https://doi.org/10.1136/lupus-2018-000285

17. Li, M., D'Arcy, C., Li, X., Zhang, T., Joober, R., & Meng, X. (2019). What do DNA methylation studies tell us about depression? A systematic review. Translational psychiatry, 9(1), 68. https://doi.org/10.1038/s41398-019-0412-y

18. Łoboś, P., & Regulska-Ilow, B. (2021). Link between methyl nutrients and the DNA methylation process in the course of selected diseases in adults. Roczniki Panstwowego Zakladu Higieny, 72(2), 123–136. https://doi.org/10.32394/rpzh.2021.0157

19. Lorincz, A. T. (2016). Virtues and Weaknesses of DNA Methylation as a Test for Cervical Cancer Prevention. Acta Cytologica, 60(6), 501–512. https://doi.org/10.1159/000450595

20. Loyfer, N., Magenheim, J., Peretz, A., Cann, G., Bredno, J., Klochendler, A., Fox-Fisher, I., Shabi-Porat, S., Hecht, M., Pelet, T., Moss, J., Drawshy, Z., Amini, H., Moradi, P., Nagaraju, S., Bauman, D., Shveiky, D., Porat, S., Dior, U., Rivkin, G., … Kaplan, T. (2023). A DNA methylation atlas of normal human cell types. Nature, 613(7943), 355–364. https://doi.org/10.1038/s41586-022-05580-6

21. Mandaviya, P. R., Joehanes, R., Brody, J., Castillo-Fernandez, J. E., Dekkers, K. F., Do, A. N., Graff, M., Hänninen, I. K., Tanaka, T., de Jonge, E. A. L., Kiefte-de Jong, J. C., Absher, D. M., Aslibekyan, S., de Rijke, Y. B., Fornage, M., Hernandez, D. G., Hurme, M. A., Ikram, M. A., Jacques, P. F., Justice, A. E., … Heil, S. G. (2019). Association of dietary folate and vitamin B-12 intake with genome-wide DNA methylation in blood: a large-scale epigenome-wide association analysis in 5841 individuals. The American journal of clinical nutrition, 110(2), 437–450. https://doi.org/10.1093/ajcn/nqz031

22. Martínez-Iglesias, O., Carrera, I., Carril, J. C., Fernández-Novoa, L., Cacabelos, N., & Cacabelos, R. (2020). DNA Methylation in Neurodegenerative and Cerebrovascular Disorders. International journal of molecular sciences, 21(6), 2220. https://doi.org/10.3390/ijms21062220

23. Matthews, R. (2022, October 10). MTHFR Gene Variants: Diagnosis & Treatment. Rupa Health. https://www.rupahealth.com/post/mthfr-gene-variants-diagnosis-treatment

24. Mayo Clinic Staff. (2017). SAMe. Mayo Clinic. https://www.mayoclinic.org/drugs-supplements-same/art-20364924

25. Menezo, Y., Clement, P., Clement, A., & Elder, K. (2020). Methylation: An Ineluctable Biochemical and Physiological Process Essential to the Transmission of Life. International journal of molecular sciences, 21(23), 9311. https://doi.org/10.3390/ijms21239311

26. Methylation Panel | Rupa Health Patient Help Center. (n.d.). Support.rupahealth.com. Retrieved March 1, 2024, from https://support.rupahealth.com/en/articles/8552298-methylation-panel

27. Methylation Panel by Genova Diagnostics. (n.d.). Rupa Health. Retrieved March 1, 2024, from https://www.rupahealth.com/lab-tests/genova-methylation-panel#biomarkers

28. Moll, S., & Varga, E. A. (2015). Homocysteine and MTHFR Mutations. Circulation, 132(1). https://doi.org/10.1161/circulationaha.114.013311

29. MTHFR Mutation Test: MedlinePlus Medical Test. (n.d.). Medlineplus.gov. Retrieved March 1, 2024, from https://medlineplus.gov/lab-tests/mthfr-mutation-test/#:~:text=To%20lower%20homocysteine%20levels%2C%20your

30. Paul, C., & Brady, D. M. (2017). Comparative Bioavailability and Utilization of Particular Forms of B12 Supplements With Potential to Mitigate B12-related Genetic Polymorphisms. Integrative medicine (Encinitas, Calif.), 16(1), 42–49.

31. Persaud, N. S., & Cates, H. M. (2022). The Epigenetics of Anxiety Pathophysiology: A DNA Methylation and Histone Modification Focused Review. Eneuro, 10(4), ENEURO.0109-21.2021. https://doi.org/10.1523/eneuro.0109-21.2021

32. Phillips, T. (2008). The Role of Methylation in Gene Expression | Learn Science at Scitable. Nature.com. https://www.nature.com/scitable/topicpage/the-role-of-methylation-in-gene-expression-1070/

33. Pouliot, M. C., Labrie, Y., Diorio, C., & Durocher, F. (2015). The Role of Methylation in Breast Cancer Susceptibility and Treatment. Anticancer research, 35(9), 4569–4574.

34. Shi, Y., Zhang, H., Huang, S., Yin, L., Wang, F., Luo, P., & Huang, H. (2022). Epigenetic regulation in cardiovascular disease: mechanisms and advances in clinical trials. Signal Transduction and Targeted Therapy, 7(1). https://doi.org/10.1038/s41392-022-01055-2

35. Smith, A. D., & Refsum, H. (2016). Homocysteine, B Vitamins, and Cognitive Impairment. Annual review of nutrition, 36, 211–239. https://doi.org/10.1146/annurev-nutr-071715-050947

36. Yang, Z., Zhang, S., Ouyang, L., Liao, A., He, Y., Li, Z., & Chen, X. (2023). DNA Methylation and Bipolar Disorder. Journal of Psychiatry and Brain Science, 8(6). https://doi.org/10.20900/jpbs.20230012

37. Yoshimura, H. (2023, December 27). Epigenetics and Disease Prevention: Harnessing Lifestyle Changes in Clinical Practice. Rupa Health. https://www.rupahealth.com/post/epigenetics-and-disease-prevention-harnessing-lifestyle-changes-in-clinical-practice

38. Yoshimura, H. (2024a, January 16). What is Methylation: An Introduction to Its Role in Health and Disease for Functional Medicine Practitioners. Rupa Health. https://www.rupahealth.com/post/what-is-methylation-an-introduction-to-its-role-in-health-and-disease-for-functional-medicine-practitioners

39. Yoshimura, H. (2024b, January 26). Nutritional Support for Optimal Methylation: A Guide for Functional Medicine. Rupa Health. https://www.rupahealth.com/post/nutritional-support-for-optimal-methylation-a-guide-for-functional-medicine

40. Yoshimura, H. (2024c, February 2). The Role of B Vitamins in Methylation Processes: Clinical Applications and Dosage Guidelines. Rupa Health. https://www.rupahealth.com/post/the-role-of-b-vitamins-in-methylation-processes-clinical-applications-and-dosage-guidelines

41. Zakhari S. (2013). Alcohol metabolism and epigenetics changes. Alcohol research : current reviews, 35(1), 6–16.

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Kidney International
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The Journal of Allergy and Clinical Immunology
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Annals of Surgery
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Chest
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Diabetes Care
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Circulation
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Nature Medicine
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Hey Practitioners! On December 11th, join Dr. Terry Wahls in a free live class where she'll share her groundbreaking methods for managing MS and autoimmune patients. This live session will address your most pressing questions and will take a closer look at treatment options beyond the conventional standards of care. Register here.

Register Here