Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Reference Guide
  /  
Immunosciences Lab, Inc.
  /  
B. burgdorferi IgG, IgM by Western Blot

B. burgdorferi IgG, IgM by Western Blot

By 
Immunosciences Lab, Inc.
B. burgdorferi IgG, IgM by Western Blot
Immunosciences Lab, Inc.
Sign up free to order the 
B. burgdorferi IgG, IgM by Western Blot
 test.
One login for 30+ lab companies.
Start an Order

About the Test

Ordering Borrelia Burgdorferi IgG, IgM by Western Blot with Immunosciences Lab

This assay is known as the tier-two blood test for the confirmation of Lyme disease with high accuracy. It detects Borrelia-specific peptide antibodies in human serum, was refined by different investigators, and was recommended for a better diagnosis of Lyme disease.

The advantage of Western Blot over the ELISA method is that while ELISA uses a mixture of proteins for testing, in WB, the antigens are separated based on their molecular size, for example, 18, 23, 28, 30, 39,

41, 45, 58, 66, and 93 kDa prior to completion of the ELISA steps. The disadvantage is that the antibody reaction with each pure antigen can only be observed qualitatively or simply a positive or negative, yes or no result.

According to the CDC/ASTPHLD Working Group:

  • An IgG blot is considered positive (reactive) if 5 of the following ten peptide bands are present: p18, p23, p28, p30, p39, p41, p45, p58, p66, p93.
  • An IgM blot is considered positive (reactive) if 2 of the following three peptide bands are present: p23, p39, and p41.
  • If the IgG or IgM antibodies against the above peptides are negative, the patient is considered non-reactive.

A sample-positive Western Blot test is shown in the figure below.

IgG and IgM antibody patterns with different peptides of Borrelia burgdorferi in patients confirmed with Lyme disease


The Western Blot (WB) assay has been widely used to detect the presence of antibodies in human serum and plasma to various infectious disease agents.

In this procedure, component proteins of purified, inactivated organisms are electrophoretically separated by SDS-polyacrylamide electrophoresis followed by electrotransfer to nitrocellulose sheets. Each strip serves as the solid-phase antigen for an ELISA test.

Specific antibodies present in human serum/plasma will bind to several of the separated polypeptides upon incubation with the strip. These antibodies are then treated with an enzyme-antibody conjugate which binds to the human immunoglobulins if present. The final product of the membrane antigen, a human antibody, and the conjugate is visualized upon incubation with a chromogenic enzyme substrate. This will result in a blue-colored “band” at the polypeptide location on the membrane strip if the specific human antibody is present.

The Borrelia burgdorferi IgG, IgM by Western Blot is Best Used For Which Patients?

This panel is beneficial for a wide variety of patients with:

  • Tick bite
  • Erythema Migrans positive
  • Unclassified autoimmune disease
  • Chronic synovitis
  • Rheumatoid arthritis
  • Lyme arthritis
  • Psoriatic arthritis
  • Lupus
  • Neuroborreliosis
  • Lymphocytic meningitis
  • Cranial neuritis
  • Painful radiculoneuritis

Symptoms of Lyme Disease

The Borrelia burgdorferi IgG, IgM by Western Blot should be considered when patients present the following symptoms and conditions:

  • Immune system-swollen glands, fever/chills, weakened immune defenses.
  • Heart- irregular heartbeat, inflammation.
  • Muscles- weakness, heartbeat, inflammation.
  • Rash- flat red rash, not itchy or painful, may have a central clearing.
  • Brain- memory loss, inability to concentrate, headache, neck pain and stiffness, encephalitis or meningitis, psychosis.
  • Eyes- blurred vision, floaters, irregular pupil.
  • Nerves- Pain, often down an arm or leg, pins and needles, tingling, burning electric shock sensations, numbness, paralysis, often on one side of the face, twitching, and spasms.
  • Joints- joint pain, inflammatory arthritis in one or several joints.

About the Test

This test confirms the presence of ongoing Lyme disease based on CDC criteria.

Lab Test Comparisons
To help you choose the best test for your patients, we’ve built lab test comparisons!
Select any lab tests to compare up to 18 key characteristics between them. You also have the ability to copy & share a link to your comparisons to anyone inside or outside of Rupa!

More Resources

No items found.

Biomarkers

No items found.

Hey providers! 👋 Join us for Fullscript Forward, a free virtual Health & Tech Summit on Friday, June 13, designed to help you level up your care with smarter tools, sharper insights, and cutting-edge strategies. Whether you're diving deeper into women’s health, optimizing supplement protocols, improving patient outcomes with adherence tools, or staying ahead with the latest in labs and diagnostics, this summit is built to support your clinical expertise and practice growth. Register Today!

Hey practitioners! 👋 Join Dr. Lisa Pomeroy, ND for an insightful webinar, "The Role of the Gut Microbiome in Brain Health," on Wednesday, June 18 at 2:00 PM EST / 11:00 AM PT.

In this session, you'll gain insight into how the gut microbiome may influence brain health through the gut-brain axis, how microbially produced substances may affect the nervous system, and what research says about GI-MAP test patterns related to cognitive and emotional well-being.

Register now to secure your spot.

Related Articles

No items found.
Start an Order
Lab Test Information
Price
$
120
.00
 $
120.00
Sign up to View
Sample Type
Serum
Shipping Time
3 - 4 days
USPS
Turnaround Time
15 days
Test Preparation Starts
No test preparation required
Number of Collection Days
1 day
Methods Used For Processing
Western Blot
Lab Certifications
CLIA Certified
CAP Accredited
ISO 15189
COLA Accredited
Order, track, and receive results from 30+ labs in one place.