Borrelia Species DNA, Real-Time PCR, with Reflexes, Blood - The diagnosis of Borreliosis is most often made by clinical examination combined with evidence of tick bite or exposure in endemic areas. Amplification of Borrelia genomic DNA from blood, fluids or tissues can support the diagnosis. This test detects Borrelia species which are associated with either Lyme disease (Borrelia burgdorferi), or relapsing fever (Borrelia miyamotoi).