Folate, Serum - This test checks the level of folic acid in your body, which is especially important for pregnant women, people who don't eat enough raw fruits and vegetables, those with alcohol dependence, and individuals with damage to their small intestine. Sometimes, low levels of folic acid might be due to not having enough vitamin B<sub>12</sub>, which affects folate levels. Dietary supplements containing biotin may interfere in assay results. For patients supplementing with daily doses of biotin, draw samples at least 8-72 hours following the last biotin supplementation.