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Reference Guide
Diagnostic Solutions


Diagnostic Solutions
Diagnostic Solutions
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About the Test

If you want to order the Zonulin add-on, please order the "GI-MAP + Zonulin" test. The GI-MAP measures a variety of gastrointestinal microbiota DNA using qPCR technology. It detects microbial imbalance, microbes contributing to illness, and indicators of digestion, absorption, inflammation, and immune function. Due to a supply shortage, this test no longer includes antibiotic resistance genes to opportunistic/universal bacteria.

Ordering the GI-MAP® - GI Microbial Assay Plus

The GI-MAP® from Diagnostic Solutions Laboratory is a comprehensive stool analysis that measures gastrointestinal microbiota DNA from a single stool sample. Using state-of-the-art, quantitative polymerase chain reaction (qPCR) technology, the GI-MAP evaluates gut pathogens, bacterial infections, yeast overgrowth, viruses, and parasites. Antibiotic resistance genes and virulence factors are also included on the test for unparalleled insight into H. pylori.

Recently, the GI-MAP has been enhanced to include an upgraded report with visual cues that allow for easier readability. Further, additional markers, including Roseburia spp., Desulfovibrio spp., and Eosinophil Activation Protein (EPX/EDN), have been added to include even greater insight into patient health.

The GI-MAP was designed to detect microbes that may be disturbing normal microbial balance or contributing to illness. To complete the picture of overall health, the GI-MAP includes key markers related to digestion, absorption, inflammation, and immune function.

An unhealthy gut can cause uncomfortable symptoms that are felt beyond the gastrointestinal (GI) tract. In fact, the root cause of brain fog, fatigue, headaches, food sensitivities, and more can often be traced back to the gut.

The GI-MAP offers impactful, quantitative results that enable practitioners to create personalized protocols that address the underlying cause(s) of uncomfortable symptoms for even the most complex patients.

Why Is Quantification Using qPCR Technology So Important?

qPCR offers a much more accurate way to detect and quantify clinically-relevant organisms than standard PCR, culture, microscopy, or DNA sequencing-based methods. Accurately assessing how much

of an organism's DNA is present in a patient's stool sample is essential for helping practitioners to determine the clinical significance of pathogenic organisms and dysbiosis patterns.

GI-MAP Allows for the Personalized Gut-Healing Treatment Plans

The GI-MAP allows practitioners to create personalized treatment protocols to address gut dysfunction based on which infections are urgent, which areas of the gut are already optimized, and which areas should be addressed after an infection is resolved.

Additionally, the quantification offers a remarkable ability to see how treatment modalities are working because a retest after treatment can show whether a parasite has resolved, dysbiosis has improved, and more.

Who Can Benefit from a GI-MAP Comprehensive Stool Analysis Test?

Because of its comprehensive analysis of gut health, almost every patient can benefit from a GI-MAP gut health assessment. The GI-MAP is perfect for patients wishing to optimize their health, and it's a critical test for patients who have been chronically ill and frustrated without a diagnosis for years. Some conditions that warrant testing are:

  • Autoimmune diseases
  • Irritable bowel syndrome (IBS)
  • Inflammatory bowel disease (IBD)
  • Digestive complaints (bloating, diarrhea, or constipation)
  • Brain fog
  • Skin problems like acne and psoriasis
  • Mood disorders, depression, and anxiety
  • Diabetes and weight loss issues

What Sample is Needed to Complete the GI-MAP test?

The GI-MAP is analyzed from a single stool sample. The collection is easy: patients collect their stool and use the provided tools to gather some of it into a specimen vial. This vial is returned to Diagnostic Solutions via the provided prepaid envelope and then analyzed. Results are sent to your Rupa Health portal!

About the Test

The GI-MAP is a comprehensive stool test that utilizes qPCR technology to detect parasites, bacteria, fungi, and more, allowing practitioners to create personalized treatment protocols to address gut dysfunction.


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Lab Test Information
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Sample Type
Shipping Time
2 - 3 days
Turnaround Time
10 days
Test Preparation Starts
No test preparation required
Number of Collection Days
1 day
Methods Used For Processing
Lab Certifications
CLIA Certified
CAP Accredited
ISO 15189
COLA Accredited
Order, track, and receive results from 30+ labs in one place.